DHM

Optical system for digital holographic microscopy and determination of optical properties of bulk media (DHM)

General information

Description
Optical system for digital holographic microscopy and determination of optical properties of bulk media
Acquisition year
2021
Contact person
Prof. Miran Bürmen, Ph.D.
Co-financier
Slovenian Research and Innovation Agency (ARIS)

Optical system for digital holographic microscopy

The digital holographic microscopy system includes an optical table with active vibration damping and a modular inverted optical microscope with accessories for performing an interferometric layout for digital holographic microscopy. The optical microscope is fully motorized and allows, through two platforms, a direct access to the optical path and thus the possibility of external extensions with different optical systems. Basically, optical microscopy enables bright field microscopy with Köller illumination, phase contrast and differential interference contrast microscopy, epifluorescence microscopy and polarization microscopy. The optical microscope accessories include a wide range of optical lenses, optical adapters and adapters for fixing various samples. The extension for digital holographic microscopy includes two high-coherence laser systems, a wavefront modulation system, a scanning system and modular optical components for optical path adjustment, and the implementation of interferometric systems on an optical table.

Important components

  1. Optical table top: Standa HT15-24-40

    1. dimensions of the table top: 2400 x 1500 mm

    2. thickness of the table top: 400 mm

    3. height of the table top: 1000 mm

    4. active base with pneumatic vibration damping

  2. Motorized inverted optical microscope: Nikon Eclipse Ti2-E

    1. two platforms

    2. acquisition techniques: bright field, differential interference contrast, phase contrast, polarization microscopy, epi-fluorescence

  3. Extension for digital holographic microscopy

    1. several manufacturers / suppliers (Midowlark, Thorlabs, Edmund Optics, Ximea, Elveflow, ScannerMax, CNI Lasers)

    2. wavefront modulator

    3. scanning system

    4. high coherence length laser systems: 532 nm or 632.8 nm

    5. fast camera: 4000 fps at HD resolution

    6. piezo pumps and flow microchannels for suspensions and liquid samples

Access to equipment

Access to the equipment is possible only by prior arrangement and at a time when the equipment is not occupied by conducting ongoing research.

Price list

The cost of using the system depends on the complexity of sample preparation and the associated material costs for sample preparation. The informative price of using the equipment with the operator is 200 EUR/h.

Determination of optical properties of bulk media

The system for the determination of the optical properties of bulk media includes the determination of the refractive index with a refractometer, and the determination of the scattering properties and the absorption coefficient by spatially distinguished reflectance spectroscopy, which is performed using a multi-channel spectrometer system. The refractometer enables accurate and reproducible measurement of the refractive index at several wavelengths. Measurements can be made on solid elastic as well as on liquid samples, while precisely controlling the temperature of the sample, which can considerably affect the dispersion curve. The multi-channel spectrometer system enables computer-synchronized and fast capture of light spectra supplied by optical fibers. The sensitivity of spectrometers comprises the ultraviolet, visible and near-infrared regions of the spectrum.

Important components

  1. Spectrometers: Avantes AVASPEC-ULS2048CL-EVO-RS-UA

    1. wavelength range: 200 to 1000 nm

    2. interchangeable inlet slots: 25, 50, 100 and 200 µm

    3. SMA fiber optic connectors

  2. Refractometer: Schmidt & Haensch ATR-L

    1. refractive index measurements: at 400, 490, 589, 660, 750, 850 and 950 nm

    2. refractive index measuring range: from 1.33200 to 1.70000

    3. resolution: 0.00001

    4. accuracy: ±0.00004

    5. changing and controlling the sample temperature: from 10 to 80°C

Access to equipment

Access to the equipment is possible only by prior arrangement and at a time when the equipment is not occupied by conducting ongoing research.

Price list

The cost of using the system depends on the complexity of sample preparation and the associated material costs for sample preparation. The informative price of using the equipment with the operator is 200 EUR/h.
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